Biological <i>in Vitro</i> and <i>in Vivo</i> Studies of the Milk Thistle Seed Oil

The milk thistle plant is one of the famous plants that have been gaining popularity for its therapeutic potential centuries. Milk seed oil (MTSO) has subjected to extensive research. fixed was extracted from seeds Silybum marianum (L.) using petroleum ether as a solvent by soxhlet device. GC-MS used identify chemical composition oil. antioxidant activity MTSO tested ABTS method, which showed ability inhibit lipid peroxidation. In addition, antimicrobial and antifungal investigations were examined. It proved an inhibitory effect against Gram-positive bacteria (Staphylococcus aureus), Gram-negative (Escherichia coli), effects (Candida albicans). slightly higher effectiveness fungi than bacteria. Moreover, cytotoxic activities on hepatocellular colorectal carcinoma shown moderate HCT-116 cell line weak HePG-2. Whereas; in vivo study done five diagnosed patients who impaired liver function, recruited study. Their weight ranged 100 ± 30 kg, their age range between 39 - 50 years. Each patient given ten drops daily added little water four-week period. improve function injured liver. present work aims oil, estimating pharmacological properties This focused showing importance our lives source antioxidants, anti-bacterial anti-fungal, anti-cancer colon. also sheds light treatment liver, fatty due improvement all markers, so it can be hired effective human therapy.